Mouse Primary Diabetic Dermal Microvascular Endothelial Cells

Catalog No.  MD-6064

Suggested Medium: Endothelial Cell Medium /w Kit – 500 ml

Catalog No.  M1168

Product Description

Mouse Primary Diabetic Dermal Microvascular Endothelial Cells from Cell Biologics are isolated from the skin of Diabetic (db/db) mice. Mouse Primary Diabetic Dermal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10⁶ cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

Mouse Primary Diabetic Dermal Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Mouse Primary Diabetic Dermal Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

Mouse Primary Diabetic Dermal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Biologics 是一家国际领先的原代细胞和细胞培养耗材供应商，提供多领域高品质的人源或动物原代细胞、包括内皮细胞、上皮细胞、肿瘤和干细胞，并提供优化的细胞培养基和其他相关产品。Cell Biologics 还首次提供基因修饰动物来源如鼠源的原代内皮细胞，以及根据用户特殊的科研需求订制细胞。公司同时发展靶向自身免疫疾病的治疗平台和新型临床生物制剂的输送系统。

主要产品线：

原代细胞：小鼠原代细胞、基因修饰小鼠原代细胞（客户订制）、人源原代细胞、大鼠原代细胞、疾病研究动物模型的原代细胞、猕猴原代细胞、兔原代细胞、猪原代细胞、肿瘤细胞、其他细胞（犬、仓鼠）、GFP表达的小鼠内皮细胞（客户定制）

细胞培养基和试剂：细胞基础培养基、细胞培养基、经典细胞培养基、细胞完全培养基、培养基试剂盒、细胞培养相关试剂

肿瘤细胞系：人肿瘤细胞、小鼠肿瘤细胞

干细胞：大鼠间充质干细胞、干细胞培养基、小鼠骨髓祖细胞、小鼠胚胎干细胞、小鼠间充质干细胞

动物组织器官：小鼠组织和器官、小鼠体液、大鼠组织和器官、大鼠体液

分子生物学：大鼠及小鼠蛋白裂解液，RNA, DNA

其他产品（犬、仓鼠细胞）：犬原代细胞、仓鼠原代细胞、GFP表达小鼠内皮细胞