C57BL/6-GFP Mouse Primary Small Intestinal Fibroblasts

Catalog No. mGFP-6025

Suggested Medium: Fibroblast Medium /w Kit - 500 ml

Catalog No. M2267

Product Description

C57BL/6-GFP Mouse Primary Small Intestinal Fibroblasts from Cell Biologics are isolated from C57BL/6-Tg(CAG-EGFP)1Osb/J mouse small intestinal tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Primary Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen. 

Product Testing

C57BL/6-GFP Mouse Primary Intestinal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. C57BL/6-GFP Mouse Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

C57BL/6-GFP Mouse Primary Intestinal Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics Products

C57BL/6-GFP Mouse Primary Intestinal Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Although C57BL/6-GFP Mouse Intestinal Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Biologics 是一家国际领先的原代细胞和细胞培养耗材供应商，提供多领域高品质的人源或动物原代细胞、包括内皮细胞、上皮细胞、肿瘤和干细胞，并提供优化的细胞培养基和其他相关产品。Cell Biologics 还首次提供基因修饰动物来源如鼠源的原代内皮细胞，以及根据用户特殊的科研需求订制细胞。公司同时发展靶向自身免疫疾病的治疗平台和新型临床生物制剂的输送系统。

主要产品线：

原代细胞：小鼠原代细胞、基因修饰小鼠原代细胞（客户订制）、人源原代细胞、大鼠原代细胞、疾病研究动物模型的原代细胞、猕猴原代细胞、兔原代细胞、猪原代细胞、肿瘤细胞、其他细胞（犬、仓鼠）、GFP表达的小鼠内皮细胞（客户定制）

细胞培养基和试剂：细胞基础培养基、细胞培养基、经典细胞培养基、细胞完全培养基、培养基试剂盒、细胞培养相关试剂

肿瘤细胞系：人肿瘤细胞、小鼠肿瘤细胞

干细胞：大鼠间充质干细胞、干细胞培养基、小鼠骨髓祖细胞、小鼠胚胎干细胞、小鼠间充质干细胞

动物组织器官：小鼠组织和器官、小鼠体液、大鼠组织和器官、大鼠体液

分子生物学：大鼠及小鼠蛋白裂解液，RNA, DNA

其他产品（犬、仓鼠细胞）：犬原代细胞、仓鼠原代细胞、GFP表达小鼠内皮细胞